3ozz

X-ray diffraction
1.7Å resolution

Structure of a cytochrome b5 core-swap mutant

Released:
Source organism: Bos taurus
Entry authors: Terzyan S, Parthasarathy S, Zhang XC, Benson D

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cytochrome b5 Chain: B
Molecule details ›
Chain: B
Length: 82 amino acids
Theoretical weight: 9.45 KDa
Source organism: Bos taurus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P00171 (Residues: 8-89; Coverage: 61%)
Gene names: CYB5, CYB5A
Sequence domains: Cytochrome b5-like Heme/Steroid binding domain
Structure domains: Cytochrome b5-like heme/steroid binding domain

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH3R
Spacegroup: C2
Unit cell:
a: 110.669Å b: 30.654Å c: 23.553Å
α: 90° β: 95.65° γ: 90°
R-values:
R R work R free
0.176 0.176 0.229
Expression system: Escherichia coli BL21