3oqz

X-ray diffraction
2.5Å resolution

Semi-synthetic ribonuclease S: meta-cyano-phenylalanine at position 8

Released:
Source organism: Bos taurus
Primary publication:
Nitrile bonds as infrared probes of electrostatics in ribonuclease S.
J Phys Chem B 114 13536-44 (2010)
PMID: 20883003

Function and Biology Details

Reaction catalysed:
(1a) an (RNA) containing cytidine = an (RNA)-3'-cytidine-2',3'-cyclophosphate + a 5'-hydroxy-ribonucleotide-3'-(RNA)
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
PDBe Complex ID:
PDB-CPX-158391 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Ribonuclease pancreatic Chains: a, b
Molecule details ›
Chains: a, b
Length: 15 amino acids
Theoretical weight: 1.78 KDa
Source organism: Bos taurus
Expression system: Not provided
UniProt:
  • Canonical: P61823 (Residues: 27-41; Coverage: 12%)
Gene names: RNASE1, RNS1
Ribonuclease pancreatic Chains: A, B
Molecule details ›
Chains: A, B
Length: 104 amino acids
Theoretical weight: 11.56 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P61823 (Residues: 47-150; Coverage: 84%)
Gene names: RNASE1, RNS1
Sequence domains: Pancreatic ribonuclease
Structure domains: Ribonuclease A-like domain

Ligands and Environments

No bound ligands
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: C2
Unit cell:
a: 99.85Å b: 31.679Å c: 69.171Å
α: 90° β: 90.93° γ: 90°
R-values:
R R work R free
0.207 0.204 0.268
Expression system: Not provided