› 3ol3

X-ray diffraction
1.95Å resolution

Crystal structure of a putative uncharacterized protein from Mycobacterium smegamtis, an ortholog of Rv0543c, iodide phased

Released:
DOI: 10.2210/pdb3ol3/pdb
PDB entry 3ol3 coloured by chain, front view.
PDB entry 3ol3 coloured by chain, side view.
PDB entry 3ol3 coloured by chain, top view.
Source organism: Mycolicibacterium smegmatis MC2 155
Primary publication:
Structural diversity in the Mycobacteria DUF3349 superfamily.
Buchko GW, Abendroth J, Robinson JI, Phan IQ, Myler PJ, Edwards TE
Protein Sci 29 670-685 (2020)
PMID: 31658388

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-106276 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
EndoIII-related endonuclease Chains: A, B
Molecule details ›
Chains: A, B
Length: 107 amino acids
Theoretical weight: 11.7 KDa
Source organism: Mycolicibacterium smegmatis MC2 155
Expression system: Escherichia coli
UniProt:
  • Canonical: A0QRC4 (Residues: 1-103; Coverage: 100%)
Gene name: MSMEG_1066
Sequence domains: Protein of unknown function (DUF3349)
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand IOD 21 x IOD
Ligand PG4 1 x PG4
Ligand PGE 2 x PGE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU FR-E+ SUPERBRIGHT
Spacegroup: P21212
Unit cell:
a: 71.87Å b: 72.9Å c: 45.14Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.198 0.197 0.236
Expression system: Escherichia coli

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Citations

3 mentions without citation

SAD phasing using iodide ions in a high-throughput structural genomics environment.
Abendroth et al. (2011)
2 more