3oha

X-ray diffraction
2Å resolution

Yeast DNA polymerase eta inserting dCTP opposite an 8oxoG lesion

Released:

Function and Biology Details

Reaction catalysed:
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA polymerase eta Chain: A
Molecule details ›
Chain: A
Length: 520 amino acids
Theoretical weight: 58.81 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: Q04049 (Residues: 1-513; Coverage: 81%)
Gene names: DBH1, RAD30, YDR419W
Sequence domains: impB/mucB/samB family
Structure domains:
5'-D(*GP*TP*CP*CP*TP*CP*CP*CP*CP*TP*(DOC))-3' Chain: P
Molecule details ›
Chain: P
Length: 11 nucleotides
Theoretical weight: 3.21 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Not provided
5'-D(P*TP*(8OG)P*GP*AP*GP*GP*GP*GP*AP*GP*GP*AP*C)-3' Chain: T
Molecule details ›
Chain: T
Length: 13 nucleotides
Theoretical weight: 4.14 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Not provided

Ligands and Environments

3 bound ligands:
2 modified residues:

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: C2221
Unit cell:
a: 88.039Å b: 227.635Å c: 86.077Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.159 0.157 0.195
Expression systems:
  • Escherichia coli
  • Not provided