PDBe 3ogm

X-ray diffraction
3.34Å resolution

Structure of COI1-ASK1 in complex with coronatine and the JAZ1 degron

Released:

Function and Biology Details

Structure analysis Details

Assemblies composition:
hetero trimer (preferred)
hetero dimer
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
SKP1-like protein 1A Chains: A, C, E, G, I, K, M, O
Molecule details ›
Chains: A, C, E, G, I, K, M, O
Length: 160 amino acids
Theoretical weight: 17.88 KDa
Source organism: Arabidopsis thaliana
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q39255 (Residues: 1-160; Coverage: 100%)
Gene names: ASK1, At1g75950, SKP1, SKP1A, T4O12.17, UIP1
Sequence domains:
Structure domains: Potassium Channel Kv1.1; Chain A
Coronatine-insensitive protein 1 Chains: B, D, F, H, J, L, N, P
Molecule details ›
Chains: B, D, F, H, J, L, N, P
Length: 592 amino acids
Theoretical weight: 67.75 KDa
Source organism: Arabidopsis thaliana
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: O04197 (Residues: 1-592; Coverage: 100%)
Gene names: At2g39940, COI1, FBL2, T28M21.10
Sequence domains:
Structure domains:
Protein TIFY 10A Chains: Q, R, S, U, V, W, X
Molecule details ›
Chains: Q, R, S, U, V, W, X
Length: 21 amino acids
Theoretical weight: 2.6 KDa
Source organism: Arabidopsis thaliana
Expression system: Not provided
UniProt:
  • Canonical: Q9LMA8 (Residues: 200-220; Coverage: 8%)
Gene names: At1g19180, JAZ1, T29M8.5, TIFY10A

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P21
Unit cell:
a: 123.17Å b: 220.758Å c: 149.54Å
α: 90° β: 104.45° γ: 90°
R-values:
R R work R free
0.227 0.226 0.27
Expression systems:
  • Spodoptera frugiperda
  • Not provided