3o9x

X-ray diffraction
2.1Å resolution

Structure of the E. coli antitoxin MqsA (YgiT/b3021) in complex with its gene promoter

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Antitoxin MqsA Chains: A, B
Molecule details ›
Chains: A, B
Length: 133 amino acids
Theoretical weight: 14.92 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q46864 (Residues: 1-131; Coverage: 100%)
Gene names: JW2989, b3021, mqsA, ygiT
Sequence domains: Antitoxin component of bacterial toxin-antitoxin system, MqsA
Structure domains:
DNA (26-MER) Chain: F
Molecule details ›
Chain: F
Length: 26 nucleotides
Theoretical weight: 7.97 KDa
Source organism: Escherichia coli K-12
Expression system: Not provided
DNA (26-MER) Chain: E
Molecule details ›
Chain: E
Length: 26 nucleotides
Theoretical weight: 8 KDa
Source organism: Escherichia coli K-12
Expression system: Not provided

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P41
Unit cell:
a: 60.996Å b: 60.996Å c: 148.612Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.181 0.176 0.228
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided