3o4o

X-ray diffraction
3.3Å resolution

Crystal structure of an Interleukin-1 receptor complex

Released:
Source organism: Homo sapiens
Primary publication:
Structural insights into the assembly and activation of IL-1β with its receptors.
Nat. Immunol. 11 905-11 (2010)
PMID: 20802483

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Interleukin-1 beta Chain: A
Molecule details ›
Chain: A
Length: 158 amino acids
Theoretical weight: 17.81 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P01584 (Residues: 117-269; Coverage: 57%)
Gene names: IL1B, IL1F2
Sequence domains: Interleukin-1 / 18
Structure domains: Trefoil (Acidic Fibroblast Growth Factor, subunit A)
Interleukin-1 receptor type 2, soluble form Chain: C
Molecule details ›
Chain: C
Length: 339 amino acids
Theoretical weight: 38.95 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P27930 (Residues: 14-343; Coverage: 86%)
Gene names: IL1R2, IL1RB
Sequence domains:
Structure domains: Immunoglobulins
Interleukin-1 receptor accessory protein Chain: B
Molecule details ›
Chain: B
Length: 339 amino acids
Theoretical weight: 39.26 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q9NPH3 (Residues: 21-350; Coverage: 60%)
  • Best match: Q9NPH3-2 (Residues: 21-350)
Gene names: C3orf13, IL1R3, IL1RAP
Sequence domains:
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P212121
Unit cell:
a: 44.83Å b: 177.47Å c: 180.66Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.253 0.252 0.289
Expression systems:
  • Escherichia coli BL21(DE3)
  • Spodoptera frugiperda