PDBe 3n97

X-ray diffraction
3.25Å resolution

RNA polymerase alpha C-terminal domain (E. coli) and sigma region 4 (T. aq. mutant) bound to (UP,-35 element) DNA

Released:
Entry authors: Lara-Gonzalez S, Birktoft JJ, Lawson CL

Function and Biology Details

Reaction catalysed:
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Entry contents:
2 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (4 distinct):
RNA polymerase sigma factor SigA Chains: A, D
Molecule details ›
Chains: A, D
Length: 72 amino acids
Theoretical weight: 8.53 KDa
Source organism: Thermus aquaticus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9EZJ8 (Residues: 366-438; Coverage: 16%)
Gene name: sigA
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain
DNA-directed RNA polymerase subunit alpha Chains: B, C
Molecule details ›
Chains: B, C
Length: 84 amino acids
Theoretical weight: 9.36 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A7Z4 (Residues: 246-329; Coverage: 26%)
Gene names: JW3257, b3295, pez, phs, rpoA, sez
Structure domains: 5' to 3' exonuclease, C-terminal subdomain
DNA (5'-D(*TP*GP*GP*AP*AP*AP*AP*AP*AP*GP*TP*AP*CP*TP*TP*GP*AP*CP*AP*TP*GP*G)-3') Chain: M
Molecule details ›
Chain: M
Length: 22 nucleotides
Theoretical weight: 6.85 KDa
DNA (5'-D(*CP*CP*AP*TP*GP*TP*CP*AP*AP*GP*TP*AP*CP*TP*TP*TP*TP*TP*TP*CP*C)-3') Chain: N
Molecule details ›
Chain: N
Length: 21 nucleotides
Theoretical weight: 6.34 KDa

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: C2221
Unit cell:
a: 80.71Å b: 86.41Å c: 147.11Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.257 0.254 0.295
Expression system: Escherichia coli