3n70

X-ray diffraction
2.8Å resolution

The Crystal Structure of the P-loop NTPase domain of the Sigma-54 transport activator from E. coli to 2.8A

Released:
Source organism: Escherichia coli CFT073
Entry authors: Stein AJ, Mulligan R, Volkart L, Freeman L, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Transport activator Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 145 amino acids
Theoretical weight: 16.64 KDa
Source organism: Escherichia coli CFT073
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0H2VDI2 (Residues: 143-287; Coverage: 35%)
Gene names: c5204, pgtA
Sequence domains: Sigma-54 interaction domain
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

1 bound ligand:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P43
Unit cell:
a: 77.612Å b: 77.612Å c: 215.088Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.232 0.229 0.28
Expression system: Escherichia coli BL21(DE3)