› 3mz9

X-ray diffraction
1.8Å resolution

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152624 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nickel-binding periplasmic protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 502 amino acids
Theoretical weight: 56.36 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P33590 (Residues: 23-524; Coverage: 100%)
Gene names: JW3441, b3476, nikA
Sequence domains: Bacterial extracellular solute-binding proteins, family 5 Middle
Structure domains:

Ligands and Environments

7 bound ligands:
Ligand FE 2 x FE
Ligand BHN 2 x BHN
Ligand DTD 2 x DTD
Ligand CL 4 x CL
Ligand GOL 13 x GOL
Ligand ACT 8 x ACT
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P212121
Unit cell:
a: 87.083Å b: 93.848Å c: 124.731Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.184 0.182 0.219
Expression system: Escherichia coli

Quick links

Citations

8 review citations

Protein design: toward functional metalloenzymes.
Yu et al. (2014)
7 more