PDBe 3mwq

X-ray diffraction
1.68Å resolution

Crystal structure of ribonuclease A tandem enzymes and their interaction with the cytosolic ribonuclease inhibitor

Released:

Function and Biology Details

Reaction catalysed:
(1a) an (RNA) containing cytidine = an (RNA)-3'-cytidine-2',3'-cyclophosphate + a 5'-hydroxy-ribonucleotide-3'-(RNA)
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribonuclease pancreatic Chain: A
Molecule details ›
Chain: A
Length: 256 amino acids
Theoretical weight: 28.15 KDa
Source organism: Bos taurus
Expression system: Escherichia coli
UniProt:
  • Canonical: P61823 (Residues: 27-150, 26-150; Coverage: 100%)
Gene names: RNASE1, RNS1
Sequence domains: Pancreatic ribonuclease
Structure domains: Ribonuclease A-like domain

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007
Spacegroup: C2
Unit cell:
a: 101.632Å b: 32.915Å c: 73.391Å
α: 90° β: 91.03° γ: 90°
R-values:
R R work R free
0.176 0.174 0.216
Expression system: Escherichia coli