› 3mth

X-ray diffraction
1.9Å resolution

X-RAY CRYSTALLOGRAPHIC STUDIES ON HEXAMERIC INSULINS IN THE PRESENCE OF HELIX-STABILIZING AGENTS, THIOCYANATE, METHYLPARABEN AND PHENOL

Released:
DOI: 10.2210/pdb3mth/pdb
PDB entry 3mth coloured by chain, front view.
PDB entry 3mth coloured by chain, side view.
PDB entry 3mth coloured by chain, top view.
Source organism: Sus scrofa
Primary publication:
X-ray crystallographic studies on hexameric insulins in the presence of helix-stabilizing agents, thiocyanate, methylparaben, and phenol.
Whittingham JL, Chaudhuri S, Dodson EJ, Moody PC, Dodson GG
Biochemistry 34 15553-63 (1995)
PMID: 7492558

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
hetero dodecamer
hetero tetramer
hetero hexamer
Assembly name:
PDBe Complex ID:
PDB-CPX-134237 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Insulin A chain Chains: A, C
Molecule details ›
Chains: A, C
Length: 21 amino acids
Theoretical weight: 2.38 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: P01315 (Residues: 88-108; Coverage: 25%)
Gene name: INS
Insulin B chain Chains: B, D
Molecule details ›
Chains: B, D
Length: 30 amino acids
Theoretical weight: 3.4 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: P01315 (Residues: 25-54; Coverage: 36%)
Gene name: INS
Sequence domains: Insulin/IGF/Relaxin family

Ligands and Environments

3 bound ligands:
Ligand MPB 1 x MPB
Ligand ZN 2 x ZN
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: R3
Unit cell:
a: 80.38Å b: 80.38Å c: 37Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.184 not available not available

Quick links

Citations

4 review citations

Zinc-ligand interactions modulate assembly and stability of the insulin hexamer -- a review.
Dunn MF. (2005)
3 more

12 mentions without citation

LIGSITEcsc: predicting ligand binding sites using the Connolly surface and degree of conservation.
Huang et al. (2006)
11 more