PDBe 3msy

X-ray diffraction
2.5Å resolution

Crystal Structure of Mandelate racemase/muconate lactonizing enzyme from a Marine actinobacterium

Released:
Entry authors: Satyanarayana L, Burley SK, Swaminathan S, New York SGX Research Center for Structural Genomics (NYSGXRC)

Function and Biology Details

Reaction catalysed:
(+)-muconolactone = cis,cis-muconate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
MR_MLE domain-containing protein Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 379 amino acids
Theoretical weight: 43.09 KDa
Source organism: marine actinobacterium PHSC20C1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A4AFX2 (Residues: 20-388; Coverage: 95%)
Gene name: A20C1_08358
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P212121
Unit cell:
a: 92.003Å b: 103.393Å c: 234.641Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.266 0.238 0.268
Expression system: Escherichia coli BL21(DE3)