3ml4

X-ray diffraction
2.6Å resolution

Crystal structure of a complex between Dok7 PH-PTB and the MuSK juxtamembrane region

Released:

Function and Biology Details

Reaction catalysed:
ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate
Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Protein Dok-7 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 224 amino acids
Theoretical weight: 25.1 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q18PE0 (Residues: 1-220; Coverage: 44%)
Gene name: Dok7
Sequence domains: PTB domain (IRS-1 type)
Structure domains: Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)
Muscle, skeletal receptor tyrosine-protein kinase Chains: E, F, G, H
Molecule details ›
Chains: E, F, G, H
Length: 13 amino acids
Theoretical weight: 1.75 KDa
Source organism: Mus musculus
Expression system: Not provided
UniProt:
  • Canonical: Q61006 (Residues: 544-556; Coverage: 2%)
Gene names: Musk, Nsk2

Ligands and Environments

No bound ligands

2 modified residues:

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4C
Spacegroup: P3221
Unit cell:
a: 134.6Å b: 134.6Å c: 121.58Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.255 0.253 0.302
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided