3mkr

X-ray diffraction
2.6Å resolution

Crystal structure of yeast alpha/epsilon-COP subcomplex of the COPI vesicular coat

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
Non-polymer only dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Coatomer subunit epsilon Chain: A
Molecule details ›
Chain: A
Length: 291 amino acids
Theoretical weight: 33.11 KDa
Source organism: Bos taurus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q28104 (Residues: 17-307; Coverage: 95%)
Gene names: COPE, COPE1
Structure domains: Serine Threonine Protein Phosphatase 5, Tetratricopeptide repeat
Coatomer subunit alpha Chain: B
Molecule details ›
Chain: B
Length: 320 amino acids
Theoretical weight: 36.31 KDa
Source organism: Bos taurus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q27954 (Residues: 905-1224; Coverage: 26%)
Gene name: COPA

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E, NSLS BEAMLINE X25
Spacegroup: I422
Unit cell:
a: 176.629Å b: 176.629Å c: 141.177Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.222 0.222 0.263
Expression system: Escherichia coli BL21(DE3)