3mhv

X-ray diffraction
3.1Å resolution

Crystal Structure of Vps4 and Vta1

Released:
Source organism: Saccharomyces cerevisiae
Primary publication:
Structural role of the Vps4-Vta1 interface in ESCRT-III recycling.
Structure 18 976-84 (2010)
PMID: 20696398

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Vacuolar protein sorting-associated protein 4 Chain: C
Molecule details ›
Chain: C
Length: 117 amino acids
Theoretical weight: 12.81 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P52917 (Residues: 299-413; Coverage: 26%)
Gene names: CSC1, DID6, END13, GRD13, P9705.10, VPL4, VPS4, VPT10, YPR173C
Structure domains: Helicase, Ruva Protein; domain 3
Vacuolar protein sorting-associated protein VTA1 Chain: A
Molecule details ›
Chain: A
Length: 42 amino acids
Theoretical weight: 4.71 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: Q06263 (Residues: 289-330; Coverage: 13%)
Gene names: VTA1, YLR181C
Sequence domains: Vta1 C-terminal domain
Structure domains: Immunoglobulin FC, subunit C

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P2221
Unit cell:
a: 37.979Å b: 70.322Å c: 88.839Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.311 0.298 0.317
Expression system: Escherichia coli