PDBe 3m6l

X-ray diffraction
1.59Å resolution

Crystal structure of transketolase in complex with thiamine diphosphate, ribose-5-phosphate and calcium ion

Released:
Entry authors: Nocek B, Makowska-Grzyska M, Maltseva N, Joachimiak A, Anderson W, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Reaction catalysed:
Sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate = D-ribose 5-phosphate + D-xylulose 5-phosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Transketolase Chain: A
Molecule details ›
Chain: A
Length: 635 amino acids
Theoretical weight: 70.42 KDa
Source organism: Campylobacter jejuni subsp. jejuni NCTC 11168 = ATCC 700819
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q0P7Y3 (Residues: 1-632; Coverage: 100%)
Gene names: Cj1645, tkt
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand TPP 1 x TPP

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: C2
Unit cell:
a: 130.924Å b: 71.462Å c: 69.617Å
α: 90° β: 109.48° γ: 90°
R-values:
R R work R free
0.18 0.16 0.201
Expression system: Escherichia coli BL21(DE3)