PDBe 3m55

X-ray diffraction
1.55Å resolution

SET7/9 Y305F in complex with TAF10-K189me1 peptide and AdoHcy

Released:

Function and Biology Details

Reaction catalysed:
S-adenosyl-L-methionine + L-lysine-[histone] = S-adenosyl-L-homocysteine + N(6)-methyl-L-lysine-[histone]
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Histone-lysine N-methyltransferase SETD7 Chain: A
Molecule details ›
Chain: A
Length: 261 amino acids
Theoretical weight: 29.03 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8WTS6 (Residues: 110-366; Coverage: 70%)
Gene names: KIAA1717, KMT7, SET7, SET9, SETD7
Sequence domains: SET domain
Structure domains:
Transcription initiation factor TFIID subunit 10 Chain: B
Molecule details ›
Chain: B
Length: 11 amino acids
Theoretical weight: 1.27 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q12962 (Residues: 186-195; Coverage: 5%)
Gene names: TAF10, TAF2A, TAF2H, TAFII30

Ligands and Environments


Cofactor: Ligand SAH 1 x SAH
No bound ligands

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: P3221
Unit cell:
a: 83.524Å b: 83.524Å c: 95.673Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.192 0.191 0.217
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided