PDBe 3m4c

X-ray diffraction
1.9Å resolution

A Zn-mediated tetrahedral protein lattice cage encapsulating a microperoxidase

Released:
Primary publication:
Structural characterization of a microperoxidase inside a metal-directed protein cage.
Angew. Chem. Int. Ed. Engl. 49 7014-8 (2010)
PMID: 20721993

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assemblies composition:
hetero pentamer (preferred)
hetero 15-mer
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Heme-Peptide Fragment Chain: E
Molecule details ›
Chain: E
Length: 9 amino acids
Theoretical weight: 1.01 KDa
Source organism: Synthetic construct
Expression system: Not provided
Soluble cytochrome b562 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 106 amino acids
Theoretical weight: 11.65 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0ABE7 (Residues: 23-128; Coverage: 100%)
Gene name: cybC
Sequence domains: Cytochrome b562
Structure domains: Four Helix Bundle (Hemerythrin (Met), subunit A)

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: R32
Unit cell:
a: 126.892Å b: 126.892Å c: 167.944Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.19 0.187 0.223
Expression systems:
  • Not provided
  • Escherichia coli