PDBe 3m3n

X-ray diffraction
7Å resolution

Structure of a Longitudinal Actin Dimer Assembled by Tandem W Domains

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Actin, alpha skeletal muscle Chains: A, B
Molecule details ›
Chains: A, B
Length: 375 amino acids
Theoretical weight: 41.88 KDa
Source organism: Oryctolagus cuniculus
UniProt:
  • Canonical: P68135 (Residues: 3-377; Coverage: 100%)
Gene names: ACTA, ACTA1
Sequence domains: Actin
Neural Wiskott-Aldrich syndrome protein; Thymosin beta-4 Chain: W
Molecule details ›
Chain: W
Length: 101 amino acids
Theoretical weight: 11.09 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P20065 (Residues: 24-50; Coverage: 54%)
  • Canonical: Q91YD9 (Residues: 397-472; Coverage: 15%)
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Gene names: Ptmb4, Tmsb4, Tmsb4x, Wasl
Sequence domains: WH2 motif

Ligands and Environments

2 bound ligands:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 17-BM
Spacegroup: P6522
Unit cell:
a: 100.743Å b: 100.743Å c: 458.839Å
α: 90° β: 90° γ: 120°
Expression system: Escherichia coli