› 3m1c

X-ray diffraction
3Å resolution

Crystal structure of the conserved herpesvirus fusion regulator complex gH-gL

Released:
DOI: 10.2210/pdb3m1c/pdb
PDB 3m1c coloured by chain and viewed from the front.
PDB 3m1c coloured by chain and viewed from the side.
PDB 3m1c coloured by chain and viewed from the top.
Source organism: Human herpesvirus 2 strain HG52
Primary publication:
Crystal structure of the conserved herpesvirus fusion regulator complex gH-gL.
Chowdary TK, Cairns TM, Atanasiu D, Cohen GH, Eisenberg RJ, Heldwein EE
OpenAccess logo Nat. Struct. Mol. Biol. 17 882-8 (2010)
PMID: 20601960

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (3 distinct):
Envelope glycoprotein H Chain: A
Molecule details ›
Chain: A
Length: 762 amino acids
Theoretical weight: 81.63 KDa
Source organism: Human herpesvirus 2 strain HG52
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P89445 (Residues: 48-803; Coverage: 92%)
Gene names: UL22, gH
Structure domains:
Envelope glycoprotein L Chain: B
Molecule details ›
Chain: B
Length: 204 amino acids
Theoretical weight: 23.19 KDa
Source organism: Human herpesvirus 2 strain HG52
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P28278 (Residues: 21-224; Coverage: 98%)
Gene names: UL1, gL
Sequence domains: Herpesvirus glycoprotein L
Structure domains: Enolase-like; domain 1

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
2 bound ligands:
Ligand NAG 2 x NAG
Ligand XYL 1 x XYL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P41212
Unit cell:
a: 88.255Å b: 88.255Å c: 333.406Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.176 0.17 0.242
Expression system: Spodoptera frugiperda

Quick links

Citations

26 review citations

Herpes Simplex Virus Cell Entry Mechanisms: An Update.
Madavaraju et al. (2020)
25 more

18 mentions without citation

gH/gL supercomplexes at early stages of herpesvirus entry.
Heldwein EE. (2016)
17 more