PDBe 3lx0

X-ray diffraction
1.5Å resolution

Crystal structure of Staphylococcal nuclease variant Delta+PHS D21N at cryogenic temperature

Source organism: Staphylococcus aureus
Entry authors: Doctrow BM, Schlessman JL, Garcia-Moreno EB, Heroux A

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Nuclease A Chain: A
Molecule details ›
Chain: A
Length: 143 amino acids
Theoretical weight: 16.14 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli
  • Best match: P00644 (Residues: 83-231)
Gene name: nuc

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P21
Unit cell:
a: 30.745Å b: 60.379Å c: 34.531Å
α: 90° β: 97.76° γ: 90°
R R work R free
0.181 0.179 0.222
Expression system: Escherichia coli