3luh

X-ray diffraction
2Å resolution

Crystal structure of MID domain from hAGO2 in complex with GMP

Released:

Function and Biology Details

Reaction catalysed:
Endonucleolytic cleavage to 5'-phosphomonoester
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein argonaute-2 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 138 amino acids
Theoretical weight: 15.33 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9UKV8 (Residues: 439-575; Coverage: 16%)
Gene names: AGO2, EIF2C2
Structure domains: Rossmann fold

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P1
Unit cell:
a: 40.433Å b: 46.971Å c: 66.352Å
α: 86.06° β: 72.77° γ: 83.7°
R-values:
R R work R free
0.188 0.185 0.233
Expression system: Escherichia coli