PDBe 3lev

X-ray diffraction
2.5Å resolution

HIV-1 antibody 2F5 in complex with epitope scaffold ES2

Released:
Source organisms:
Primary publication:
Elicitation of structure-specific antibodies by epitope scaffolds.
Proc. Natl. Acad. Sci. U.S.A. 107 17880-7 (2010)
PMID: 20876137

Function and Biology Details

Structure analysis Details

Assemblies composition:
hetero trimer (preferred)
monomeric
hetero dimer
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
RNA polymerase sigma factor SigA Chain: A
Molecule details ›
Chain: A
Length: 179 amino acids
Theoretical weight: 20.21 KDa
Source organism: Thermus aquaticus
Expression system: Homo sapiens
UniProt:
  • Canonical: Q9EZJ8 (Residues: 93-271; Coverage: 41%)
Gene name: sigA
Sequence domains: Sigma-70 factor, region 1.2
Structure domains: Four Helix Bundle (Hemerythrin (Met), subunit A)
2F5 ANTIBODY LIGHT CHAIN Chain: L
Molecule details ›
Chain: L
Length: 214 amino acids
Theoretical weight: 23.31 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins
2F5 ANTIBODY HEAVY CHAIN Chain: H
Molecule details ›
Chain: H
Length: 237 amino acids
Theoretical weight: 25.23 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Structure domains: Immunoglobulins

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P212121
Unit cell:
a: 58.506Å b: 63.933Å c: 200.019Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.206 0.201 0.253
Expression system: Homo sapiens