PDBe 3l95

X-ray diffraction
2.19Å resolution

Crystal structure of the human Notch1 Negative Regulatory Region (NRR) bound to the fab fragment of an antagonist antibody

Released:

Function and Biology Details

Structure analysis Details

Assemblies composition:
hetero pentamer (preferred)
hetero tetramer
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
anti-NRR1 fab fragment light chain Chains: A, L
Molecule details ›
Chains: A, L
Length: 214 amino acids
Theoretical weight: 23.34 KDa
Source organism: Homo sapiens
Expression system: Not provided
Structure domains: Immunoglobulins
anti-NRR1 fab fragment heavy chain Chains: B, H
Molecule details ›
Chains: B, H
Length: 227 amino acids
Theoretical weight: 24.24 KDa
Source organism: Homo sapiens
Expression system: Not provided
Structure domains: Immunoglobulins
Notch 1 extracellular truncation Chains: X, Y
Molecule details ›
Chains: X, Y
Length: 244 amino acids
Theoretical weight: 27.1 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P46531 (Residues: 1448-1728; Coverage: 9%)
Gene names: NOTCH1, TAN1
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P212121
Unit cell:
a: 46.254Å b: 163.934Å c: 179.62Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.227 0.222 0.271
Expression systems:
  • Not provided
  • Escherichia coli