3l4f

X-ray diffraction
2.8Å resolution

Crystal Structure of betaPIX Coiled-Coil Domain and Shank PDZ Complex

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
Non-polymer only tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Rho guanine nucleotide exchange factor 7 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 61 amino acids
Theoretical weight: 7.33 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: O55043 (Residues: 587-646; Coverage: 9%)
Gene names: Arhgef7, Pak3bp, Pixb
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces
SH3 and multiple ankyrin repeat domains protein 1 Chain: D
Molecule details ›
Chain: D
Length: 132 amino acids
Theoretical weight: 14.62 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9WV48 (Residues: 653-765; Coverage: 5%)
  • Best match: Q9WV48-2 (Residues: 41-151)
Gene name: Shank1
Sequence domains: PDZ domain
Structure domains: Pdz3 Domain

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 4A, PHOTON FACTORY BEAMLINE BL-5A
Spacegroup: P3212
Unit cell:
a: 47.666Å b: 47.666Å c: 263.161Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.272 0.272 0.307
Expression system: Escherichia coli