PDBe 3l0x

X-ray diffraction
3Å resolution

Structure of split yeast PCNA

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Proliferating cell nuclear antigen Chain: A
Molecule details ›
Chain: A
Length: 169 amino acids
Theoretical weight: 19.24 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P15873 (Residues: 1-163; Coverage: 63%)
Gene names: POL30, YBR0811, YBR088C
Sequence domains: Proliferating cell nuclear antigen, N-terminal domain
Structure domains: DNA Polymerase III, subunit A, domain 2
Proliferating cell nuclear antigen Chain: B
Molecule details ›
Chain: B
Length: 94 amino acids
Theoretical weight: 10.42 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P15873 (Residues: 165-258; Coverage: 36%)
Gene names: POL30, YBR0811, YBR088C
Structure domains: DNA Polymerase III; Chain A, domain 2

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 4.2.2
Spacegroup: P213
Unit cell:
a: 122.999Å b: 122.999Å c: 122.999Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.244 0.243 0.267
Expression system: Escherichia coli