PDBe 3l0w

X-ray diffraction
2.8Å resolution

Structure of split monoubiquitinated PCNA with ubiquitin in position two

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Proliferating cell nuclear antigen Chain: A
Molecule details ›
Chain: A
Length: 169 amino acids
Theoretical weight: 19.24 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P15873 (Residues: 1-163; Coverage: 63%)
Gene names: POL30, YBR0811, YBR088C
Sequence domains: Proliferating cell nuclear antigen, N-terminal domain
Structure domains: DNA Polymerase III, subunit A, domain 2
Proliferating cell nuclear antigen; Ubiquitin Chain: B
Molecule details ›
Chain: B
Length: 169 amino acids
Theoretical weight: 18.71 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P0CG63 (Residues: 153-228; Coverage: 20%)
  • Canonical: P15873 (Residues: 165-255; Coverage: 35%)
  • nullnull
Gene names: POL30, SCD2, UBI4, YBR0811, YBR088C, YLL039C
Sequence domains: Ubiquitin family
Structure domains:

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 4.2.2
Spacegroup: P213
Unit cell:
a: 122.516Å b: 122.516Å c: 122.516Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.279 0.277 0.314
Expression system: Escherichia coli