PDBe 3kvv

X-ray diffraction
1.8Å resolution

Trapping of an oxocarbenium ion intermediate in UP crystals

Released:

Function and Biology Details

Reaction catalysed:
Uridine + phosphate = uracil + alpha-D-ribose 1-phosphate 
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Uridine phosphorylase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 253 amino acids
Theoretical weight: 27.19 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P12758 (Residues: 1-253; Coverage: 100%)
Gene names: JW3808, b3831, udp
Sequence domains: Phosphorylase superfamily
Structure domains: Rossmann fold

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 8-BM
Spacegroup: P32
Unit cell:
a: 92.864Å b: 92.864Å c: 145.016Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.175 0.173 0.205
Expression system: Escherichia coli BL21(DE3)