3kov

X-ray diffraction
2.9Å resolution

Structure of MEF2A bound to DNA reveals a completely folded MADS-box/MEF2 domain that recognizes DNA and recruits transcription co-factors

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Myocyte-specific enhancer factor 2A Chains: A, B, I, J
Molecule details ›
Chains: A, B, I, J
Length: 90 amino acids
Theoretical weight: 10.63 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q02078 (Residues: 2-91; Coverage: 18%)
Gene names: MEF2, MEF2A
Sequence domains: SRF-type transcription factor (DNA-binding and dimerisation domain)
Structure domains: Transcription factor, MADS-box
DNA (5'-D(*AP*AP*CP*TP*AP*TP*TP*TP*AP*TP*AP*AP*G)-3') Chains: C, K
Molecule details ›
Chains: C, K
Length: 13 nucleotides
Theoretical weight: 3.97 KDa
Source organism: Homo sapiens
Expression system: Not provided
DNA (5'-D(*TP*CP*TP*TP*AP*TP*AP*AP*AP*TP*AP*GP*T)-3') Chains: D, L
Molecule details ›
Chains: D, L
Length: 13 nucleotides
Theoretical weight: 3.96 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: OTHER
Spacegroup: P21212
Unit cell:
a: 77.801Å b: 77.96Å c: 106.794Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.224 0.221 0.279
Expression systems:
  • Escherichia coli
  • Not provided