3kj1

X-ray diffraction
1.94Å resolution

Mcl-1 in complex with Bim BH3 mutant I2dA

Released:

Function and Biology Details

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
hetero octamer
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Induced myeloid leukemia cell differentiation protein Mcl-1 Chain: A
Molecule details ›
Chain: A
Length: 158 amino acids
Theoretical weight: 17.94 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q07820 (Residues: 172-327; Coverage: 45%)
Gene names: BCL2L3, MCL1
Sequence domains: Apoptosis regulator proteins, Bcl-2 family
Structure domains: Blc2-like
Bcl-2-like protein 11 Chain: B
Molecule details ›
Chain: B
Length: 22 amino acids
Theoretical weight: 2.76 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O43521 (Residues: 143-163; Coverage: 11%)
Gene names: BCL2L11, BIM

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: I222
Unit cell:
a: 53.066Å b: 72.007Å c: 117.845Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.188 0.187 0.213
Expression system: Escherichia coli BL21(DE3)