X-ray diffraction
2.9Å resolution

Crystal structure of Escherichia coli AlkB with Co(II) and 2-OG


Function and Biology Details

Reaction catalysed:
DNA-base-CH(3) + 2-oxoglutarate + O(2) = DNA-base + formaldehyde + succinate + CO(2)
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Alpha-ketoglutarate-dependent dioxygenase AlkB Chains: A, B
Molecule details ›
Chains: A, B
Length: 219 amino acids
Theoretical weight: 24.4 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
  • Canonical: P05050 (Residues: 1-216; Coverage: 100%)
Gene names: JW2200, aidD, alkB, b2212
Structure domains: Alpha-ketoglutarate-dependent dioxygenase AlkB-like

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: C2
Unit cell:
a: 148.624Å b: 42.806Å c: 86.454Å
α: 90° β: 121.77° γ: 90°
R R work R free
0.234 0.23 0.307
Expression system: Escherichia coli