PDBe 3kcp

X-ray diffraction
1.94Å resolution

Crystal structure of interacting Clostridium thermocellum multimodular components

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Cellulosomal-scaffolding protein A Chain: A
Molecule details ›
Chain: A
Length: 321 amino acids
Theoretical weight: 34.66 KDa
Source organism: Hungateiclostridium thermocellum ATCC 27405
Expression system: Escherichia coli
UniProt:
  • Canonical: Q06851 (Residues: 1542-1853; Coverage: 17%)
Gene names: Cthe_3077, cipA
Sequence domains:
Structure domains:
Cellulosome anchoring protein cohesin region Chain: B
Molecule details ›
Chain: B
Length: 187 amino acids
Theoretical weight: 20.57 KDa
Source organism: Hungateiclostridium thermocellum ATCC 27405
Expression system: Escherichia coli
UniProt:
  • Canonical: A3DF10 (Residues: 27-200; Coverage: 29%)
Gene name: Cthe_1307
Sequence domains: Cohesin domain
Structure domains: Immunoglobulin-like

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F1
Spacegroup: P21212
Unit cell:
a: 169.977Å b: 58.019Å c: 56.15Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.207 0.206 0.231
Expression system: Escherichia coli