3k5l

X-ray diffraction
2.7Å resolution

Crystal structure of E.coli Pol II-abasic DNA-dATP Lt(0, 3) ternary complex

Released:
Source organism: Escherichia coli
Primary publication:
Structural insight into translesion synthesis by DNA Pol II.
Cell 139 1279-89 (2009)
PMID: 20064374

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA polymerase II Chain: A
Molecule details ›
Chain: A
Length: 786 amino acids
Theoretical weight: 90.46 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P21189 (Residues: 1-783; Coverage: 100%)
Gene names: JW0059, b0060, dinA, polB
Sequence domains:
Structure domains:
DNA (5'-D(*TP*AP*TP*(3DR)P*GP*TP*AP*CP*GP*CP*TP*AP*GP*GP*CP*AP*CP*A)-3') Chain: T
Molecule details ›
Chain: T
Length: 18 nucleotides
Theoretical weight: 5.39 KDa
Source organism: Escherichia coli
Expression system: Not provided
DNA (5'-D(*GP*TP*GP*CP*CP*TP*AP*GP*CP*GP*TP*AP*(DOC))-3') Chain: P
Molecule details ›
Chain: P
Length: 13 nucleotides
Theoretical weight: 3.95 KDa
Source organism: Escherichia coli
Expression system: Not provided

Ligands and Environments

2 bound ligands:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P212121
Unit cell:
a: 79.974Å b: 101.198Å c: 121.717Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.224 0.224 0.241
Expression systems:
  • Escherichia coli
  • Not provided