PDBe 3k33

X-ray diffraction
2.4Å resolution

Crystal structure of the Phd-Doc complex

Released:

Function and Biology Details

Reaction catalysed:
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assemblies composition:
hetero octamer (preferred)
monomeric
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Protein kinase doc Chain: A
Molecule details ›
Chain: A
Length: 126 amino acids
Theoretical weight: 13.6 KDa
Source organism: Escherichia virus P1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q06259 (Residues: 1-126; Coverage: 100%)
Gene name: doc
Sequence domains: Fic/DOC family
Structure domains: Four Helix Bundle (Hemerythrin (Met), subunit A)
Antitoxin phd Chains: B, C, D
Molecule details ›
Chains: B, C, D
Length: 73 amino acids
Theoretical weight: 8.14 KDa
Source organism: Escherichia virus P1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q06253 (Residues: 1-73; Coverage: 100%)
Gene name: phd
Sequence domains: Antitoxin Phd_YefM, type II toxin-antitoxin system
Structure domains: YefM-like domain
Polypeptide of unknown amino acids and source Chain: E
Molecule details ›
Chain: E
Length: 12 amino acids
Theoretical weight: 1.04 KDa
Source organism: unidentified
Expression system: unidentified

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7B
Spacegroup: P3221
Unit cell:
a: 48.295Å b: 48.295Å c: 347.321Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.258 0.257 0.285
Expression systems:
  • Escherichia coli BL21(DE3)
  • unidentified