3k1r

X-ray diffraction
2.3Å resolution

Structure of harmonin NPDZ1 in complex with the SAM-PBM of Sans

Released:
Source organism: Homo sapiens

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Harmonin Chain: A
Molecule details ›
Chain: A
Length: 192 amino acids
Theoretical weight: 21.96 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q9Y6N9 (Residues: 1-192; Coverage: 35%)
Gene names: AIE75, USH1C
Sequence domains: PDZ domain
Structure domains:
Usher syndrome type-1G protein Chain: B
Molecule details ›
Chain: B
Length: 74 amino acids
Theoretical weight: 8.41 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q495M9 (Residues: 388-461; Coverage: 16%)
Gene names: SANS, USH1G
Sequence domains: SAM domain (Sterile alpha motif)
Structure domains: Transcription Factor, Ets-1

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU ULTRAX 18
Spacegroup: P21
Unit cell:
a: 59.603Å b: 57.775Å c: 59.505Å
α: 90° β: 104.63° γ: 90°
R-values:
R R work R free
0.213 0.21 0.256
Expression system: Escherichia coli BL21