› 3jse

X-ray diffraction
2.9Å resolution

Crystal structure of archaeal 20S proteasome in complex with mutated P26 activator

Released:
DOI: 10.2210/pdb3jse/pdb
PDB entry 3jse coloured by chain, front view.
PDB entry 3jse coloured by chain, side view.
PDB entry 3jse coloured by chain, top view.
Source organisms:
Primary publication:
Structural models for interactions between the 20S proteasome and its PAN/19S activators.
Stadtmueller BM, Ferrell K, Whitby FG, Heroux A, Robinson H, Myszka DG, Hill CP
J Biol Chem 285 13-7 (2010)
PMID: 19889631

Function and Biology Details

Reaction catalysed: 
Cleavage of peptide bonds with very broad specificity.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 42-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-150393 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Proteasome subunit alpha Chains: A, B, C, D, E, F, G
Molecule details ›
Chains: A, B, C, D, E, F, G
Length: 227 amino acids
Theoretical weight: 25.13 KDa
Source organism: Thermoplasma acidophilum
Expression system: Escherichia coli
UniProt:
  • Canonical: P25156 (Residues: 7-233; Coverage: 97%)
Gene names: Ta1288, psmA
Sequence domains:
Structure domains: Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1
Proteasome subunit beta Chains: H, I, J, K, L, M, N
Molecule details ›
Chains: H, I, J, K, L, M, N
Length: 203 amino acids
Theoretical weight: 22.29 KDa
Source organism: Thermoplasma acidophilum
Expression system: Escherichia coli
UniProt:
  • Canonical: P28061 (Residues: 9-211; Coverage: 96%)
Gene names: Ta0612, psmB
Sequence domains: Proteasome subunit
Structure domains: Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1
Proteasome activator PA28 C-terminal domain-containing protein Chains: O, P, Q, R, S, T, U
Molecule details ›
Chains: O, P, Q, R, S, T, U
Length: 228 amino acids
Theoretical weight: 25 KDa
Source organism: Trypanosoma brucei
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9U8G2 (Residues: 4-231; Coverage: 99%)
Sequence domains: Proteasome activator pa28 beta subunit
Structure domains: Proteasome activator pa28, C-terminal domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: C2
Unit cell:
a: 254.701Å b: 126.099Å c: 180.393Å
α: 90° β: 92° γ: 90°
R-values:
R R work R free
0.213 0.212 0.241
Expression system: Escherichia coli

Quick links

Citations

12 review citations

Molecular architecture and assembly of the eukaryotic proteasome.
Tomko et al. (2013)
11 more

2 mentions without citation

Structural models for interactions between the 20S proteasome and its PAN/19S activators.
Stadtmueller et al. (2010)
1 more