PDBe 3jr6

X-ray diffraction
3Å resolution

Sequential reorganization of beta-sheet topology by insertion of a single strand

Released:
Source organism: Escherichia virus T4

Function and Biology Details

Reaction catalysed:
Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endolysin Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 170 amino acids
Theoretical weight: 19.22 KDa
Source organism: Escherichia virus T4
Expression system: Escherichia coli
UniProt:
  • Canonical: P00720 (Residues: 1-27, 28-164; Coverage: 100%)
Gene name: E
Sequence domains: Phage lysozyme
Structure domains: Lysozyme

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P21
Unit cell:
a: 59.227Å b: 75.915Å c: 81.547Å
α: 90° β: 94.06° γ: 90°
R-values:
R R work R free
0.261 0.25 0.32
Expression system: Escherichia coli