PDBe 3jal

Electron Microscopy
3.5Å resolution

Cryo-EM structure of GMPCPP-microtubule co-polymerized with EB3

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero 14-mer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Tubulin alpha-1B chain Chains: A, C, E, J, K, L
Molecule details ›
Chains: A, C, E, J, K, L
Length: 451 amino acids
Theoretical weight: 50.2 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: Q2XVP4 (Residues: 1-451; Coverage: 100%)
Gene name: TUBA1B
Sequence domains:
Structure domains:
Tubulin beta chain Chains: B, D, F, G, H, I
Molecule details ›
Chains: B, D, F, G, H, I
Length: 445 amino acids
Theoretical weight: 49.91 KDa
Source organism: Sus scrofa
UniProt:
  • Canonical: P02554 (Residues: 1-445; Coverage: 100%)
Sequence domains:
Structure domains:
Microtubule-associated protein RP/EB family member 3 Chains: M, N
Molecule details ›
Chains: M, N
Length: 203 amino acids
Theoretical weight: 22.87 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9UPY8 (Residues: 1-200; Coverage: 71%)
Gene name: MAPRE3
Sequence domains: Calponin homology (CH) domain
Structure domains: Calponin-like domain

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.5Å
Relevant EMDB volumes: EMD-6350
Expression system: Escherichia coli BL21(DE3)