3j96

Electron Microscopy
7.6Å resolution

Structure of 20S supercomplex determined by single particle cryoelectron microscopy (State I)

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tridecamer (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Vesicle-fusing ATPase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 747 amino acids
Theoretical weight: 82.91 KDa
Source organism: Cricetulus griseus
Expression system: Escherichia coli
UniProt:
  • Canonical: P18708 (Residues: 1-744; Coverage: 100%)
Gene name: NSF
Sequence domains:
Alpha-soluble NSF attachment protein Chains: G, H, I, J
Molecule details ›
Chains: G, H, I, J
Length: 297 amino acids
Theoretical weight: 33.38 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P54921 (Residues: 1-295; Coverage: 100%)
Gene names: Napa, Snap, Snapa
Vesicle-associated membrane protein 2 Chain: K
Molecule details ›
Chain: K
Length: 63 amino acids
Theoretical weight: 7.23 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P63045 (Residues: 28-89; Coverage: 53%)
Gene names: Syb2, Vamp2
Syntaxin-1A Chain: L
Molecule details ›
Chain: L
Length: 67 amino acids
Theoretical weight: 7.84 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P32851 (Residues: 191-256; Coverage: 23%)
Gene names: Sap, Stx1a
Synaptosomal-associated protein 25 Chain: M
Molecule details ›
Chain: M
Length: 188 amino acids
Theoretical weight: 21.26 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P60881 (Residues: 17-204; Coverage: 91%)
  • Best match: P60881-2 (Residues: 17-204)
Gene names: Snap, Snap25
Sequence domains: SNAP-25 family

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 7.601Å
Relevant EMDB volumes: EMD-6206
Expression system: Escherichia coli