PDBe 3j5s

Electron Microscopy
7.5Å resolution

EttA binds to ribosome exit site and regulates translation by restricting ribosome and tRNA dynamics

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Entry contents:
5 distinct polypeptide molecules
3 distinct RNA molecules
Macromolecules (8 distinct):
Energy-dependent translational throttle protein EttA Chain: D
Molecule details ›
Chain: D
Length: 561 amino acids
Theoretical weight: 63.36 KDa
Source organism: Escherichia coli str. K-12 substr. MG1655
Expression system: Escherichia coli str. K-12 substr. MG1655
UniProt:
  • Canonical: P0A9W3 (Residues: 1-555; Coverage: 100%)
Gene names: JW4354, b4391, ettA, yjjK
Sequence domains:
50S ribosomal protein L1 Chain: F
Molecule details ›
Chain: F
Length: 234 amino acids
Theoretical weight: 24.77 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7L0 (Residues: 1-234; Coverage: 100%)
Gene names: JW3947, b3984, rplA
Sequence domains: Ribosomal protein L1p/L10e family
50S ribosomal protein L5 Chain: G
Molecule details ›
Chain: G
Length: 178 amino acids
Theoretical weight: 20.2 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P62399 (Residues: 2-179; Coverage: 99%)
Gene names: JW3270, b3308, rplE
Sequence domains:
50S ribosomal protein L33 Chain: H
Molecule details ›
Chain: H
Length: 50 amino acids
Theoretical weight: 5.81 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7N9 (Residues: 4-53; Coverage: 91%)
Gene names: JW3611, b3636, rpmG
Sequence domains: Ribosomal protein L33
30S ribosomal protein S7 Chain: I
Molecule details ›
Chain: I
Length: 151 amino acids
Theoretical weight: 16.86 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P02359 (Residues: 2-152; Coverage: 84%)
Gene names: JW3303, b3341, rpsG
16S ribosomal RNA Chain: B
Molecule details ›
Chain: B
Length: 101 nucleotides
Theoretical weight: 32.64 KDa
Sequence domains: Bacterial small subunit ribosomal RNA
23S ribosomal RNA Chain: A
Molecule details ›
Chain: A
Length: 360 nucleotides
Theoretical weight: 116.24 KDa
Sequence domains: Bacterial large subunit ribosomal RNA
P-site tRNA FMet Chain: E
Molecule details ›
Chain: E
Length: 77 nucleotides
Theoretical weight: 24.8 KDa
Sequence domains: tRNA

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 7.5Å
Relevant EMDB volumes: EMD-5784
Expression system: Escherichia coli str. K-12 substr. MG1655