› 3j41

Electron Microscopy
25Å resolution

Pseudo-atomic model of the Aquaporin-0/Calmodulin complex derived from electron microscopy

Released:
DOI: 10.2210/pdb3j41/pdb
PDB 3j41 coloured by chain and viewed from the front.
PDB 3j41 coloured by chain and viewed from the side.
PDB 3j41 coloured by chain and viewed from the top.
Source organisms:
Primary publication:
Allosteric mechanism of water-channel gating by Ca(2+)-calmodulin.
Reichow SL, Clemens DM, Freites JA, Németh-Cahalan KL, Heyden M, Tobias DJ, Hall JE, Gonen T
OpenAccess logo Nat. Struct. Mol. Biol. (2013)
PMID: 23893133
Related structures: EMD-5679

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Lens fiber major intrinsic protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 263 amino acids
Theoretical weight: 28.24 KDa
Source organism: Ovis aries
UniProt:
  • Canonical: Q6J8I9 (Residues: 1-263; Coverage: 100%)
Gene names: AQP0, MIP
Sequence domains: Major intrinsic protein
Calmodulin-1 Chains: E, F
Molecule details ›
Chains: E, F
Length: 149 amino acids
Theoretical weight: 16.85 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P0DP23 (Residues: 1-149; Coverage: 100%)
Gene names: CALM, CALM1, CAM, CAM1
Sequence domains: EF-hand domain pair

Ligands and Environments

1 bound ligand:
Ligand CA 8 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 25Å
Relevant EMDB volumes: EMD-5679
Expression system: Escherichia coli BL21

Quick links

Citations

11 review citations

Aquaporins: More Than Functional Monomers in a Tetrameric Arrangement.
Ozu et al. (2018)
10 more

7 mentions without citation

Phosphorylation-Dependent Regulation of Mammalian Aquaporins.
Nesverova et al. (2019)
6 more