PDBe 3j0e

Electron Microscopy
9.9Å resolution

Models for the T. thermophilus ribosome recycling factor and the E. coli elongation factor G bound to the E. coli post-termination complex

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero nonamer (preferred)
Entry contents:
3 distinct polypeptide molecules
6 distinct RNA molecules
Macromolecules (9 distinct):
Elongation factor G Chain: H
Molecule details ›
Chain: H
Length: 702 amino acids
Theoretical weight: 77.42 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A6M8 (Residues: 2-703; Coverage: 100%)
Gene names: JW3302, b3340, far, fus, fusA
Sequence domains:
30S ribosomal protein S12 Chain: F
Molecule details ›
Chain: F
Length: 123 amino acids
Theoretical weight: 13.64 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7S3 (Residues: 2-124; Coverage: 99%)
Gene names: JW3304, b3342, rpsL, strA
Sequence domains: Ribosomal protein S12/S23
Ribosome-recycling factor Chain: G
Molecule details ›
Chain: G
Length: 185 amino acids
Theoretical weight: 21.03 KDa
Source organism: Thermus thermophilus
UniProt:
  • Canonical: Q9WX76 (Residues: 1-185; Coverage: 100%)
Gene names: TTHA0858, frr
Sequence domains: Ribosome recycling factor
16S ribosomal RNA Chain: E
Molecule details ›
Chain: E
Length: 18 nucleotides
Theoretical weight: 5.79 KDa
16S ribosomal RNA Chain: e
Molecule details ›
Chain: e
Length: 19 nucleotides
Theoretical weight: 6.17 KDa
23S ribosomal RNA Chain: A
Molecule details ›
Chain: A
Length: 22 nucleotides
Theoretical weight: 6.99 KDa
23S ribosomal RNA Chain: B
Molecule details ›
Chain: B
Length: 17 nucleotides
Theoretical weight: 5.46 KDa
23S ribosomal RNA Chain: C
Molecule details ›
Chain: C
Length: 13 nucleotides
Theoretical weight: 4.23 KDa
23S ribosomal RNA Chain: D
Molecule details ›
Chain: D
Length: 19 nucleotides
Theoretical weight: 6.12 KDa

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 9.9Å
Relevant EMDB volumes: EMD-1917