PDBe 3j0d

Electron Microscopy
11.1Å resolution

Models for the T. thermophilus ribosome recycling factor bound to the E. coli post-termination complex

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero 11-mer (preferred)
Entry contents:
3 distinct polypeptide molecules
8 distinct RNA molecules
Macromolecules (11 distinct):
30S ribosomal protein S12 Chain: I
Molecule details ›
Chain: I
Length: 123 amino acids
Theoretical weight: 13.64 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7S3 (Residues: 2-124; Coverage: 99%)
Gene names: JW3304, b3342, rpsL, strA
Sequence domains: Ribosomal protein S12/S23
50S ribosomal protein L11 Chain: G
Molecule details ›
Chain: G
Length: 141 amino acids
Theoretical weight: 14.76 KDa
Source organism: Escherichia coli
UniProt:
  • Canonical: P0A7J7 (Residues: 2-142; Coverage: 99%)
Gene names: JW3946, b3983, relC, rplK
Sequence domains:
Ribosome-recycling factor Chain: J
Molecule details ›
Chain: J
Length: 185 amino acids
Theoretical weight: 21.03 KDa
Source organism: Thermus thermophilus
UniProt:
  • Canonical: Q9WX76 (Residues: 1-185; Coverage: 100%)
Gene names: TTHA0858, frr
Sequence domains: Ribosome recycling factor
16S ribosomal RNA Chain: H
Molecule details ›
Chain: H
Length: 18 nucleotides
Theoretical weight: 5.79 KDa
16S ribosomal RNA Chain: h
Molecule details ›
Chain: h
Length: 19 nucleotides
Theoretical weight: 6.17 KDa
23S ribosomal RNA Chain: A
Molecule details ›
Chain: A
Length: 50 nucleotides
Theoretical weight: 16.11 KDa
23S ribosomal RNA Chain: B
Molecule details ›
Chain: B
Length: 22 nucleotides
Theoretical weight: 6.99 KDa
23S ribosomal RNA Chain: C
Molecule details ›
Chain: C
Length: 17 nucleotides
Theoretical weight: 5.46 KDa
23S ribosomal RNA Chain: D
Molecule details ›
Chain: D
Length: 13 nucleotides
Theoretical weight: 4.23 KDa
23S ribosomal RNA Chain: E
Molecule details ›
Chain: E
Length: 19 nucleotides
Theoretical weight: 6.12 KDa
23S ribosomal RNA Chain: F
Molecule details ›
Chain: F
Length: 19 nucleotides
Theoretical weight: 6.16 KDa

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 11.1Å
Relevant EMDB volumes: EMD-1915