3ipr

X-ray diffraction
2.5Å resolution

Crystal structure of the Enterococcus faecalis gluconate specific EIIA phosphotransferase system component

Released:
Source organism: Enterococcus faecalis
Primary publication:
Structure of the Enterococcus faecalis EIIA(gnt) PTS component.
Biochem. Biophys. Res. Commun. 388 626-9 (2009)
PMID: 19682976

Function and Biology Details

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
PTS EIIA type-4 domain-containing protein Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 150 amino acids
Theoretical weight: 15.8 KDa
Source organism: Enterococcus faecalis
Expression system: Escherichia coli
UniProt:
  • Canonical: Q82ZC8 (Residues: 1-150; Coverage: 100%)
Gene name: EF_3136
Sequence domains: PTS system fructose IIA component
Structure domains: Phosphotransferase system, mannose-type IIA component

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: C2
Unit cell:
a: 175.68Å b: 69.4Å c: 80.22Å
α: 90° β: 106.53° γ: 90°
R-values:
R R work R free
0.213 0.189 0.248
Expression system: Escherichia coli