3ipj

X-ray diffraction
1.2Å resolution

The crystal structure of one domain of the PTS system, IIabc component from Clostridium difficile

Released:
Source organism: Clostridioides difficile 630
Entry authors: Zhang R, Bigelow L, Cobb G, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
PTS system, sucrose-specific IIABC component Chains: A, B
Molecule details ›
Chains: A, B
Length: 95 amino acids
Theoretical weight: 10.79 KDa
Source organism: Clostridioides difficile 630
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q188R0 (Residues: 2-93; Coverage: 15%)
Gene name: CD630_04690
Sequence domains: phosphotransferase system, EIIB
Structure domains: Glucose permease domain IIB

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21
Unit cell:
a: 37.635Å b: 34.734Å c: 72.195Å
α: 90° β: 90.07° γ: 90°
R-values:
R R work R free
0.135 0.133 0.17
Expression system: Escherichia coli BL21