3in5

X-ray diffraction
3.2Å resolution

Structure of human DNA polymerase kappa inserting dATP opposite an 8-oxoG DNA lesion

Released:

Function and Biology Details

Reaction catalysed:
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA polymerase kappa Chains: A, B
Molecule details ›
Chains: A, B
Length: 508 amino acids
Theoretical weight: 58.04 KDa
Source organism: Homo sapiens
Expression system: Saccharomyces cerevisiae
UniProt:
  • Canonical: Q9UBT6 (Residues: 19-526; Coverage: 58%)
Gene names: DINB1, POLK
Sequence domains:
Structure domains:
DNA (5'-D(*GP*G*GP*GP*GP*AP*AP*GP*GP*AP*CP*TP*(DOC))-3') Chains: P, Q
Molecule details ›
Chains: P, Q
Length: 13 nucleotides
Theoretical weight: 4.07 KDa
Source organism: Homo sapiens
Expression system: Not provided
DNA (5'-D(*C*CP*TP*AP*(8OG)P*GP*AP*GP*TP*CP*CP*TP*TP*CP*CP*CP*CP*C)-3') Chains: T, U
Molecule details ›
Chains: T, U
Length: 18 nucleotides
Theoretical weight: 5.4 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

2 bound ligands:

2 modified residues:

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: C2221
Unit cell:
a: 116.846Å b: 154.48Å c: 217.293Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.227 0.225 0.274
Expression systems:
  • Saccharomyces cerevisiae
  • Not provided