X-ray diffraction
1.4Å resolution

Crystal Structure of AlkB in complex with Mn(II) and 2-oxoglutarate


Function and Biology Details

Reaction catalysed:
DNA-base-CH(3) + 2-oxoglutarate + O(2) = DNA-base + formaldehyde + succinate + CO(2)
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Alpha-ketoglutarate-dependent dioxygenase AlkB Chains: A, B
Molecule details ›
Chains: A, B
Length: 211 amino acids
Theoretical weight: 23.61 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
  • Canonical: P05050 (Residues: 12-216; Coverage: 95%)
Gene names: JW2200, aidD, alkB, b2212
Structure domains: Alpha-ketoglutarate-dependent dioxygenase AlkB-like

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4C
Spacegroup: P1
Unit cell:
a: 39.746Å b: 39.187Å c: 72.407Å
α: 77.18° β: 74.8° γ: 63.62°
R R work R free
0.182 0.182 0.207
Expression system: Escherichia coli