PDBe 3i08

X-ray diffraction
3.2Å resolution

Crystal structure of the S1-cleaved Notch1 Negative Regulatory Region (NRR)

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
hetero tetramer
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Neurogenic locus notch homolog protein 1 Chains: A, C
Molecule details ›
Chains: A, C
Length: 220 amino acids
Theoretical weight: 24.6 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P46531 (Residues: 1446-1664; Coverage: 9%)
Gene names: NOTCH1, TAN1
Sequence domains:
Structure domains: GMP Synthetase; Chain A, domain 3
Notch 1 extracellular truncation Chains: B, D
Molecule details ›
Chains: B, D
Length: 69 amino acids
Theoretical weight: 7.38 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P46531 (Residues: 1665-1733; Coverage: 3%)
Gene names: NOTCH1, TAN1
Sequence domains: NOTCH protein

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P43212
Unit cell:
a: 65.868Å b: 65.868Å c: 322.586Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.232 0.229 0.286
Expression system: Escherichia coli