X-ray diffraction
1.99Å resolution

Crystal Structure of catalytic fragment of E. coli AlaRS G237A in complex with AlaSA


Function and Biology Details

Reaction catalysed:
ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala)
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Alanine--tRNA ligase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 441 amino acids
Theoretical weight: 49.94 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
  • Canonical: P00957 (Residues: 2-442; Coverage: 50%)
Gene names: JW2667, alaS, b2697, lovB
Structure domains: Bira Bifunctional Protein; Domain 2

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: C2
Unit cell:
a: 162.928Å b: 162.186Å c: 125.713Å
α: 90° β: 90.01° γ: 90°
R R work R free
0.169 0.167 0.201
Expression system: Escherichia coli